European Journal of Health and Biology Education

European Journal of Health and Biology Education, 2019 - Volume 8 Issue 1, pp. 1-17
https://doi.org/10.12973/ejhbe.8.1.1

Published Online: 15 Jun 2019

Views: 802 | Downloads: 517

Payangka fish [Ophieleotrisaporos (Bleeker)] is one of the endemic species in Lake Tondano, Minahasa Regency, North Sulawesi. Empirically, Payangka fish is widely known by the Minahasa community. Identification of fish molecule has not been reported. This research begins with the molecular identification of Payangka fish and is developed in the form of audio-visual media for learning genetic material in high school. The topic of molecular genetic studies has one of biology learning that is considered complicated in high school. The characteristics of DNA material were abstract and were a combination of biological, physical and chemical studies. Practical activities of DNA analysis using molecular identification methods in high schools are difficult because they require sophisticated tools. Nevertheless, the concept of DNA as a key material in molecular biology is becoming a standard in the international biology curriculum. This research provides a solution by developing a molecular identification practicum carried out at the Unima FMIPA Biology laboratory and packaged in audiovisual media. Another advantage is the object of molecular identification, namely Payangka fish is an endemic species of Lake Tondano. This type of research was development research proposed by Tiangarajan, the 4D model. As the name implies, there were 4 stages in this research, namely Define, Design, Development, and Disseminate. To test the validity of this learning media, a trial is carried out. The test subjects consisted of two material experts, two instructional media experts, one subject teacher, and ten students for field trials. Data on the feasibility of this learning media product was collected by rubric and team evaluation. The collected data will then be analyzed with qualitative descriptive techniques and descriptive statistics. The results of this study were to indicate the level of eligibility or product validity is in very good criteria with a percentage of 92.87%.

DNA analysis development of audio visual media Payangka

• Agorreta, A., San Mauro, D., Schliewen, U., Van Tassell, J. L., Kovacic, M., Zardoya, R., & Ruber, L. (2013). Molecular phylogenetics of Gobioidei and phylogenetic placement of European gobies. Molecular Phylogenetics and Evolution, 69(3), 619–633. https://doi.org/10.1016/j.ympev.2013.07.017
• Ariyanto, A., Priyayi, D. F., & Dewi, L. (2011). Penggunaan media pembelajaran Biologi di Sekolah Menengah Atas (SMA) swasta salatiga [The use of Biology learning media in Salatiga Private High School (SMA)]. Bioeducation Journal of Biology Education/ Bioedukasi Jurnal Pendidikan Biologi, 9(1), 1–13.
• Astuti, E., & Nurcahyo, H. (2019). Development of biology learning media based on adobe flash to increase interest and conceptual understanding. Journal of Physics: Conference Series, 1241(1), 012050.
• Howell, M. E., Booth, C. S., Sikich, S. M., Helikar, T., Roston, R. L., Couch, B. A., & van Dijk, K. (2019). Student understanding of DNA structure–function relationships improves from using 3D learning modules with dynamic 3D printed models. Biochemistry and Molecular Biology Education, 47(3), 303-317. https://doi.org/10.1002/bmb.21234
• Hubert, N., Hanner, R., Holm, E., Mandrak, N. E., Taylor, E., Burridge, M., … & Bernatchez, L. (2008). Identifying Canadian freshwater fishes through DNA barcodes. PLoS ONE, 3(6), e2490. https://doi.org/10.1371/journal.pone.0002490
• Kearse, M., Moir, R., Wilson A., Stones-Havas, S., Cheung, M., Sturrock, S., … & Dummord, A. (2012). Geneious basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649. https://doi.org/10.1093/bioinformatics/bts199
• Kochzius, M., Seidel, C., Antoniou, A., Botla, S. K., Campo, D., Cariani, A., & Blohm, D. (2010). Identifying fishes through DNA barcodes and microarrays. PLoS ONE, 5(9), 1–15. https://doi.org/10.1371/journal.pone.0012620
• Kuguru, B., Groeneveld, J., Singh, S., & Mchomvu, B. (2019). First record of giant freshwater prawn Macrobrachium rosenbergii (de Man, 1879) from small-scale fisheries in East Africa, confirmed with DNA barcoding. BioInvasions Records, 8(2), 379-391.
• Mokosuli, Y. S. (2013). Karaktermorfologi, sumberpakandanbioaktivitasfarmakologisracunlebahmadu endemic sulawesiapisdorsatabinghamidanapisnigrocincta smith (Hymenoptera: Apidae) [Morphological characters, feed sources and pharmacological bioactivity of endemic honey bee poisons in Sulawesi apisDorsatabinghami and apisnigrocincta smith (Hymenoptera: Apidae)] (Unpublished doctoral dissertation). Universitas Sam Ratulangi,Manodo, Indenesia.
• Moleong, L.J. (2009). Metodologipenelitiankualitatif [Qualitative research methodology].Bandung, Indonesia: Alfabeta
• Nugraini, S. H., Choo, K. A., Hin, H. S., & Hoon, T. S. (2013). Students’ Feedback of e-AV Biology Website and the Learning Impact towards Biology. Procedia - Social and Behavioral Sciences, 103, 860–869. https://doi.org/10.1016/j.sbspro.2013.10.408
• Pegg, G. G., Sinclair, B., Briskey, L., & Aspden, W. J. (2006). MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia. Scientia Marina, 70(SUPPL. 2), 7–12. https://doi.org/10.3989/scimar.2006.70s27
• Pendong D. F., Mokosuli Y. S., & dan Sumampouw, H. M. (2010). Miskonsepsi Materi Genetika pada SMA di Kota Tomohon. [Laporan Penelitian] Lembaga Penelitian Universitas Negeri Manado.
• Revelson, A. M., Mokosuli, Y. S., Debby, J. J. R., Ellen, H. A., Christny, R., Nony, M., & Merry, M. (2019). Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene). Materials Science Forum, 967, 71-82. https://doi.org/10.4028/www.scientific.net/MSF.967.71
• Soeroto, B. (1988). MakanandanReproduksiIkanPayangka (OphieleotrisAporos (bleeker)) di DanauTondano [Food and Reproduction of Payangka Fish (OphieleotrisAporos (bleeker)) on Lake Tondano].InstitutPertanian Bogor, Bogor, Indenosia.
• Suddin, S., Mokosuli, Y. S., Marcelina, W., Orbanus, N., & Ardi, K. (2019). Molecular barcoding based 16S rRNA gene of Thermophilic bacteria from vulcanic sites, Linow Lake, Tomohon. Materials Science Forum, 967, 83-92. https://doi.org/10.4028/www.scientific.net/MSF.967.83
• Sugiyono (2015). Metode penelitian dan pengembangan [Research and development methods]. Bandung, Indonesia: Alfabeta.
• Sumampouw, H. M., Semuel, M. Y., & Oka, D. N. (2017). Analysis of cythochrome oxidase sub unit 1 Gene (CO1) of fruit fly (Droshophila sp.) from pineapples and application in teaching DNA in Senior high school. International Journal of Advanced Education and Research, 2(2), 71-77.
• Rosmah, S., Tindangen, M., & Rambitan, V. M. (2018). Analisis permasalahan terkait kebutuhan pengembangan perangkat pembelajaran model discovery learning untuk meningkatkan pemahaman konsep dan sikap ilmiah [Analysis of problems related to the need to develop discovery learning models to improve scientific understanding of concepts and attitudes]. Journal of Education: Theory, Research and Development/ Jurnal Pendidikan: Teori, Penelitian, Dan Pengembangan, 3(3), 322–324. https://dx.doi.org/10.17977/jptpp.v3i3.10637
• Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6,0. Molecular Biology and Evolution, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197
• Thiangarajan, S., Semmel, D. S., & Semmel, M. I. (1974). Instructional development for training teachers of expectional children: A source book. Minneapolis, MN: University of Minnesota.
• Trianto (2009). Mendesain model pembelajaran inovatif progresif [Designing progressive innovative learning models]. Jakarta, Indonesia: Prenada Media Group.
• Ward, R. D., Hanner, R., & Hebert, P. D. N. (2009). The campaign to DNA barcode all fishes, FISH-BOL. Journal of Fish Biology, 74(2), 329-356. https://doi.org/10.1111/j.1095-8649.2008.02080.x
• Zhao, L., Dong, J., Sun, C., Tian, Y., Hu, J., & Ye, X. (2019). Phylogenetic analysis of sooty grunter and other major freshwater fishes in the suborder Percoidei based on mitochondrial DNA. Mitochondrial DNA Part A, 30(2), 234-248. https://doi.org/10.1080/24701394.2018.1482283

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.